Body composition
& metabolism.
Process & characterization of the active compounds
The patented ingredient DNF-10® is an active fraction obtained after protein hydrolysis of Saccharomyces cerevisiae, a yeast species used for thousands of years in baking, fermented food and beverage manufacturing processes.
The manufacturing of DNF-10® involves enzymatic proteolysis and a selection of peptides with a molecular weight <10kDa after an ultrafiltration step. The material is then spray-dried to obtain a dried powder.
Active Compounds
Patented yeast protein hydrolysate from
Saccharomyces cerevisiae
Strain NBRC (ex. IFO) 2346
Peptide size: < 10 kDa
Formulation
Stability validated after heat treatment at 160°C/320°F for 30min.
Recommended daily dosage: 500mg
Science supporting DNF-10®
More than 10 years of research have been conducted to support the mechanism of action and clinical benefits of DNF-10® on body composition.
The mechanism of action of DNF-10® on the mediator of satiety has been studied in vivo in animal models. The benefits of the ingredient in the reduction of food intake and improvement of body composition have been confirmed with interventional clinical trials.
More than 10 articles supporting the mechanism of action and health benefits of DNF-10® have been published.
Mechanism of action
DNF-10®: decreasing food intake
Appetite is dependent on both short- and long-term mechanisms of hormonal regulation.
Serum levels of ghrelin change according to the level of fullness of our stomach when leptin, secreted over a 24-hour cycle by our adipose tissue, is regulated over the long term according to the fat mass; the more adipose tissue a healthy subject has, the higher the secretion of leptin.
Just before consumption of main meals, food intake is physiologically triggered by increased levels of ghrelin and maintained levels of leptin. DNF-10® significantly lowers ghrelin secretion before meals, and contributes to maintain the release of leptin from adipocytes.
The action of DNF-10® on those 2 hormones of appetite regulation is concomitant with a reduction in the secretion of the neuropeptide Y (NPY), the neuromodulator of appetite.
Mechanistic studies
- DNF-10® significantly lowers ghrelin secretion.
- DNF-10® significantly lowers the expression of NPY in the hypothalamus.
- The effect of DNF-10® on decreasing food intake is acute.
HED: Human Equivalent Dose
Ref: Reagan-Show et al., 2008
Clinical studies
2014
10 weeks
2017
8 weeks
54 subjects
Men and women
BMI > 25 kg/m2
Age: 20-50 years old
30 subjects
Women
BMI > 25 kg/m2
Age: 20-60 years old
Abdominal fat distribution
Computed tomography
No specific exercise and diet patterns.
Food record (Can-Pro)
Body composition
Impedance bioelectrical scale
No specific exercise and diet patterns.
Food record (Can-Pro)
SACHETS
2 x 500 mg/day
CAPSULES
2 x 250 mg/day
2014
10 weeks
2017
8 weeks
54 subjects
Men and women
BMI > 25 kg/m2
Age: 20-50 years old
30 subjects
Women
BMI > 25 kg/m2
Age: 20-60 years old
Abdominal fat distribution
Computed tomography
No specific exercise and diet patterns.
Food record (Can-Pro)
Body composition
Impedance bioelectrical scale
No specific exercise and diet patterns.
Food record (Can-Pro)
SACHETS
2 x 500 mg / day
CAPSULES
2 x 250 mg / day
Satiety enhancer
Subjects supplemented with DNF-10® decrease their caloric intake significantly after the first weeks of supplementation.
After 2 months, the daily caloric intake drops by 600 kcal.
The 3 groups of macronutrients are significantly impacted: subjects reduce their calorie counts from lipids, proteins, and carbohydrates. After a few weeks of consuming DNF-10, subjects have a reduced appetite for sweets.
Body weight loss
Anthropometric parameters
KEY OUTCOMES
Applications
Yeast protein hydrolysate from Saccharomyces cerevisiae
Peptide size: < 10kDa
Dietary supplements and functional foods
Compatible with probiotics (aw < 0,3)
An international reach
BELGIUM – BRAZIL – CANADA – CHINA – FRANCE – GERMANY – INDONESIA – ITALIA – JAPAN – MALAYSIA – PERU – PORTUGAL – SERBIA – SINGAPORE – SOLVENIA – SOUTH AFRICA – SOUTH KOREA – SPAIN – TAIWAN – THAILAND – UNITED KINGDOM – USA – VIETNAM
sachet, pouch drink, meal replacer,
bar, snack, tablet, capsule etc.
References
1 – Hong K et al.; Progr. Nutr.; 2015, 17:3:262-264
2 – Jung E. et al.; Phytother. Res.; 2009, 3(5):619-23
3 – Jung E. et al.; Nutrition; 2014, 30:25-32
4 – Jung E. et al.; Prev. Nutr. Food Sci.; 2017, 22(1):45-49
